Composite

Part:BBa_K2285016:Design

Designed by: Zhuoyang Chen   Group: iGEM17_SJTU-BioX-Shanghai   (2017-10-18)


J23119+Target1+eforRed(with RBS and Term added)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Target1 with the downstream reporter gene is hard to PCR as a whole part for the hairpin structure.When this chromoprotein is used as a reporter gene, you must consider that whether the upstream has a RBS and whether the downstream has a terminator.


Source

The Star1 system including Target1 and Antisense1 is originated from the AD1 strain (Chappell J, Takahashi MK, Lucks JB. 2015. Creating small transcription activating RNAs.)

References